Flow Cytometry Education Facilities & Resources Description For Grants
Please click on the instrument name for more information about its current configuration. You can schedule time on any instrument you have been trained to use via iLab. To schedule a sort on any of the sorters listed below, please use iLab as well, understanding that your proposed sort time is not valid until it has been approved by UFC staff, usually within one business day. (Sorts not yet approved appear in orange; approved sorts appear in purple.) Please refer to Core policies on Scheduling and Cancelation.
When planning new experiments, we recommend using the panel builder app at FluoroFinder (free registration for Pitt users).
If you experience any issues with a machine after hours or on a weekend, please use the Problem Log to report it, and we will resolve the issue as soon as we can.
Equipment
| Equipment Name | Description |
|---|---|
| Assembly ZellScanner | Canopy's ZellScanner One is a fully intergrated automated imaging system that enables quantitaitve measure of high-plex protein biomarker assays across a range of sample types. |
| Equipment Name | Description |
|---|---|
| Aurora CS (Cell Sorter) | The Aurora CS is equipped with 5 lasers - 488 (blue), 640 (red), 405 (violet), 561 (yellow-green) 355 (UV). |
| Equipment Name | Description |
|---|---|
| Attune NxT Assembly | The Attune NxT is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green), for a total of 14 fluorescent parameters, running Windows 7. Lasers: 405 100mW Trigon, 488 100mW Trigon, 561 140mW Octagon, 640 140mW Trigon. |
| Fortessa 12 | The VMI FORTESSA is equipped with 4 lasers running Diva 8/Windows 7. Lasers: 355 20mW Trigon (UV), 405 100mW Octagon (violet), 488 100mW Octagon (blue), 640 100mW Trigon (red). 15 Parameters. |
| Fortessa 7S | The FORTESSA is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green), for a total of 13 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 20mW Trigon, 561 100mW Octagon, 640 40mW Trigon. |
| Fortessa Assembly | The FORTESSA is equipped with 5 lasers 488 (blue), 640 (red), 405 (violet), 532 (Green), 355 (UV) for a total of 16 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 50mW Octagon, 532 150mW Octagon, 640 100mW Trigon, 355 20mW Trigon. |
| LSR 15 | The LSR II is equipped with 5 lasers running on Diva 8/Windows 7. Lasers: 355 60mW Trigon (UV), 405 50mW Trigon (violet), 488 20mW Trigon (blue), 532 150mW Octagon (green), 633 40mW Trigon (red). 15 Parameters. |
| LSR Assembly | The LSR II is equipped with 5 lasers running on Diva 8/Windows 7. Lasers: 355 20mW Trigon (UV), 405 50mW Trigon (violet), 488 20mW Trigon (blue), 532 150mW Octagon (green), 640 100mW Trigon (red). 15 parameters. |
| Equipment Name | Description |
|---|---|
| Aria 15 (Cell Sorter) | The Aria 15 is equipped with 5 lasers running Diva 8 on Windows 7. Lasers: 355 20mW Trigon, 405 50mW Trigon, 488 100mW Trigon, 532 150mW Octagon, 635 30mW Trigon. 15 Paramaters. |
| Aria Assembly 5-Laser (Cell Sorter) | The Aria Assembly 5 Laser is equipped with 5 lasers 488 (blue), 640 (red), 405 (violet), 552 (light green) and 355 (UV) for a total of 16 fluorescent parameters running on Diva 9/Windows 10. |
| Aria Assembly HOOD (Cell Sorter) | The Aria Asssembly Hood is equipped with 5 lasers running Diva 8/Windows 7. Lasers: 355 60mW Trigon (UV), 405 100mW Trigon (Violet), 488 100mW Trigon (Blue), 532 100mW Octagon (Green), 640 100mW Trigon (Red). 15 Paramaters. |
| Equipment Name | Description |
|---|---|
| Analysis Computer | Analysis Copmuter for Spectroflo, FlowJo, and IDEAS software |
| ImageStream | Imaging Cytopmeter: Lasers: 488nm 200mW - 561nm 200mW - 375nm 70mW - 405nm 175mW - 642nm 150mW - 785nm Dark Field Laser |
| Equipment Name | Description |
|---|---|
| Aurora Black | 64 Fluorescence channels: 355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels; 640nm = 8 channels; 561nm = 10 channels. Efficient spectrum capture for dyes emitting in the 360nm-900nm range. No filter changes required for any fluorochromes. |
| Aurora Blue | 64 Fluorescence channels: 355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels; 640nm = 8 channels; 561nm = 10 channels. Efficient spectrum capture for dyes emitting in the 360nm-900nm range. No filter changes required for any fluorochromes. |
| Aurora Pink | 64 Fluorescence channels: 355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels; 640nm = 8 channels; 561nm = 10 channels. Efficient spectrum capture for dyes emitting in the 360nm-900nm range. No filter changes required for any fluorochromes. |
| Aurora Yellow | 64 Fluorescence channels: 355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels; 640nm = 8 channels; 561nm = 10 channels. Efficient spectrum capture for dyes emitting in the 360nm-900nm range. No filter changes required for any fluorochromes. |