The LSR II is equipped with 5 lasers running on Diva 8/Windows 7. Lasers: 355 60mW Trigon (UV), 405 50mW Trigon (violet), 488 20mW Trigon (blue), 532 150mW Octagon (green), 633 40mW Trigon (red). 15 Parameters.
Location: BST - E1514
Name in FluoroFinder: LSR II BST E1514
| Detector name | LP Mirror | BP Filter | Fluorochromes |
|---|---|---|---|
| 488 Laser A | 635 | 695/40 | PerCP/PerCP-Cy5.5 |
| 488 Laser B | 505 | 515/20 | FITC/GFP |
| 488 Laser C | --- | 488/10 | SSC |
| 532 Laser A | 755 | 780/60 | PE-Cy7 |
| 532 Laser B | 685 | 710/50 | PE-Cy5.5 |
| 532 Laser C | 635 | 670/30 | PE-Cy5 |
| 532 Laser D | 595 | 610/20 | PETxRED/PI |
| 532 Laser E | --- | 575/20 | PE |
| 640 Laser A | 755 | 780/60 | APC-Cy7, APC-H7 |
| 640 Laser B | 700 | 720/20 | Alexa 700 |
| 640 Laser C | --- | 660/20 | APC |
| 405 Laser A | 595 | 610/20 | BV605 |
| 405 Laser B | 505 | 525/50 | V500, BV510 |
| 405 Laser C | --- | 450/50 | Pacific Blue, BV421,V450 |
| 355 Laser A | 690 | 740/35 | BUV 737 |
| 505 | 530/30 | Indo-1, Hoechst | |
| 355 Laser B | --- | 379/28 | BUV 395 |
| 405/20 | Indo-1 High | ||
| 450/50 | DAPI, Indo-1 low |
Core Facility
Unified Flow Core Facility
Location
Upper Campus
Equipment Type
Conventional Flow Analyzer