The FORTESSA is equipped with 5 lasers 488 (blue), 640 (red), 405 (violet), 532 (Green), 355 (UV) for a total of 16 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 50mW Octagon, 532 150mW Octagon, 640 100mW Trigon, 355 20mW Trigon.
Location: Assembly - G530
Name in FluoroFinder: Fortessa Assembly G530
The FORTESSA is equipped with 5 lasers 488 (blue), 640 (red), 405 (violet), 532 (Green), 355 (UV) for a total of 16 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 50mW Octagon, 532 150mW Octagon, 640 100mW Trigon, 355 20mW Trigon.
| Detector name | LP Mirror | BP Filter | Fluorochromes |
|---|---|---|---|
| 532 Laser A | 750 | 780/60 | PE-CY7 |
| 532 Laser B | 670 | 695/40 | PE-CY5.5 |
| 532 Laser C | 635 | 670/30 | PE-Cy5 |
| 532 Laser D | 600 | 610/20 | PE-TxRED,ECD |
| 532 Laser E | --- | 575/26 | PE |
| 640 Laser A | 785 | 780/60 | APC-Cy7 |
| 640 Laser B | 735 | 720/20 | Alexa 700 |
| 640 Laser C | --- | 660/20 | APC |
| 405 Laser A | 595 | 610/20 | BV605 |
| 405 Laser B | 505 | 525/50 | V500 |
| 405 Laser C | --- | 450/50 | Pacific Blue |
| 488 Laser A | 685 | 710/50 | PerCP Cy5.5 |
| 488 Laser B | 655 | 670/30 | PerCP |
| 488 Laser C | 505 | 530/30 (515/20) | FITC (GFP) |
| 488 Laser D | --- | 488/10 | SSC |
| 355 Laser A | 690 | 740/35 | BUV 737 |
| 505 | 530/30 | Hoechst | |
| 355 Laser B | --- | 379/28 | BUV 395 |
| 450/50 | DAPI, Alexa 350 |
Core Facility
Unified Flow Core Facility
Location
Upper Campus
Equipment Type
Conventional Flow Analyzer