Services
- FLEX Single Cell Fixed RNA Profiling
Measure gene activity on a cell-by-cell basis and characterize transcriptomes of complex cell populations on fixed human or mouse samples with this probe-based assay.
What it Assays: Chromium Single Cell Gene Expression Flex allows you to uncover critical cellular insights from fixed samples (human and mouse). Innovative probe design enables sensitive, robust performance from whole tissue, FFPE samples, and samples with fragmented RNA. Multiplexing options are available.
- 3’ Gene Expression
Measure gene activity on a cell-by-cell basis and characterize transcriptomes of complex cell populations.
What it Assays: The Chromium Single Cell Gene Expression Solution provides a comprehensive, scalable solution for cell characterization and gene expression profiling of hundreds to tens of thousands of cells. The latest improvements allow you to detect even more unique transcripts per cell. Multiplexing options are available.
- 5’ Gene Expression
Measure the activity of immune cells and their targets.
What it Assays: The Chromium Single Cell Immune Profiling Solution is a comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and immune repertoires of hundreds to tens of thousands of T and B cells in human or mouse on a cell-by-cell basis (TCR and BCR profiling). With the addition of Feature Barcoding technology, you can also detect and analyze additional cellular readouts, such as cell surface markers and antigen specificity, to enhance immune cell phenotyping and study dynamic interactions between lymphocytes and target cells. Multiplexing options are available.
- ATAC Seq
Measure epigenetics by detecting open chromatin regions.
What it Assays: The Chromium Single Cell ATAC (Assay for Transposase Accessible Chromatin) Solution accelerates the understanding of the regulatory landscape of the genome, thereby providing insights into cell variability. The chromatin profiling of tens of thousands of single cells in parallel allows researchers to see how chromatin compaction and DNA-binding proteins regulate gene expression at high resolution.
- Multiome ATAC and Gene Expression
Obtain transcriptome and open chromatin information from the same cell. Gene expression in this system is detected in nuclei, i.e., single nuclear RNA-seq and thus the resulting transcriptomes will look different from single cell RNA-sequencing studies.
What it Assays: Simultaneously profile gene expression and open chromatin from the same cell with Chromium Single Cell Multiome ATAC + Gene Expression. Multiply your power of discovery to characterize cell types and states, and uncover gene regulatory programs.- CITE Seq
RNA sequencing that provides quantitative and qualitative information of cell surface proteins along with gene expression. Barcoded antibodies bind to individual cells, attaching a marker to the mRNA sequence that carries over through the library preparation.
Barcoded antibodies bind to surface markers on individual cells (similar to flow cytometry), the barcode marker sequence carries over through the library preparation, permitting quantitative assessment of surface antibody binding to cells along with their transcriptome information. Hundreds of surface antigens can be assayed simultaneously.Available antibodies and panels can be found at Biolegend: https://www.biolegend.com/en-us/totalseq
- BCR Library
Characterizing the BCR repertoire and linking BCR sequences to specific cell subsets. (compatible with all 5’ gene expression libraries)
Pair heavy and light chain immunoglobulin (Ig) sequences from individual B cells with full isotype resolution
Assemble and annotate full-length V(D)J gene sequences- TCR Library
Characterizing the TCR repertoire and linking TCR sequences to specific cell subsets. (compatible with all 5’ gene expression libraries)
Pair α and β chain TCR sequences from individual T cells
Assemble and annotate full-length V(D)J gene sequences
Link full-length, paired TCR α and β chain sequences with TCR-pMHC specificity- Bioinformatics
We offer sequencing and analysis for your single cell data, including sequence mapping, clustering, violin plots, dot plots, BCR and TCR clonotype analysis, proteomics analysis (Cite-seq) and open chromatin analysis (scATAC-seq). For more information, please contact us here.
