The timsTOF Pro 2 is a high-resolution accurate mass instrument that combines trapped ion mobility (TIMs) with quadrupole and time-of-flight mass analyzers. TIMs and parallel accumulation serial fragmentation (PASEF®) technology provide extremely high MS/MS speed and sensitivity using minimal sample amounts and PASeR real-time database identification. This MS is coupled to a Bruker nanoElute LC and is used for proteomic applications, such as label-free quantitation.