2016 – 2026 (citing S10 grants)
Zhang X, Guseinov AA, Jenkins L, Zhou J, Gossen F, Wang P, Al Mahmud Z, Li Y, Mahardhika AB, Müller CE, Feng M, Russell AJ, Tikhonova IG, Milligan G, Zhang C. (2026) Mechanism of GPR84 allosteric modulation at a helix 8-proximate site. Sci Adv 12, eaeh5849. PMCID: PMC13600253. doi: 10.1126/sciadv.aeh5849
Blauwkamp J, Rajaram K, Staggers SR, Harrigan O, Doud EH, Xu W, Ke H, Prigge ST, Sun SY, Absalon S. (2025) An Essential Adaptor for Apicoplast Fission and Inheritance in Malaria Parasites. (2025) Nat Commun 16, 11325. PMCID: PMC12722261. DOI: 10.1038/s41467-025-66393-5
Belford AK, Maurer JB, Duda RL, Huet A, Conway JF. (2025) Structural insights into scaffold-guided assembly of the Pseudomonas phage D3 capsid. Nat Commun 16, 11586. PMCID: PMC12749128. DOI: 10.1038/s41467-025-66648-1
Zhang X, Guseinov AA, Jenkins L, Valentini A, Marsango S, Schultz-Knudsen K, Ulven T, Rexen Ulven E, Tikhonova IG, Milligan G, Zhang C. (2025) Allosteric modulation and biased signalling at free fatty acid receptor 2. Nature 643, 1428-1438. PMCID: PMC12570966. DOI: 10.1038/s41586-025-09186-6
Mittal A, Martin MF, Provins L, Hall A, Ledecq M, Wolff C, Gillard M, Horanyi PS, Coleman JA. (2025) Mechanisms underlying allosteric modulation of antiseizure medication binding to synaptic vesicle protein 2A. Proc Natl Acad Sci U S A. 122, e2510239122. PMCID: PMC12435242. DOI: 10.1073/pnas.2510239122
Hansen KH, Golcuk M, Byeon CH, Tunc1 A, Plechinger EB, Dueholm MKD, Conway JF, Andreasen M, Gur M, Akbey Ü. (2025) Structural basis of Pseudomonas biofilm-forming functional amyloid FapC formation. Sci Adv 11, eadx7829. PMCID: PMC12459412. DOI: 10.1126/sciadv.adx7829
Freeman KG, Mondal S, Macale LS, White SJ, Podgorski J, Silva B, Ortiz V, Huet A, Narsico JT, Ho M-C, Lowary T, Conway JF, Park D, Hatfull GF. (2025) Structure and infection dynamics of mycobacteriophage Bxb1. Cell, S0092-8674(25)00345-9. PMCID: PMC12124961. DOI: 10.1016/j.cell.2025.03.027
Wieteska Ł, Taylor AB, Punch E, Coleman JA, Conway IO, Lin YF, Byeon CH, Hinck CS, Krzysiak T, Ishima R, López-Casillas F, Cherepanov P, Bernard DJ, Hill CS, Hinck AP. (2025) Structures of TGF-β with betaglycan and signaling receptors reveal mechanisms of complex assembly and signaling. Nat Commun 16, 1778. PMCID: PMC11865472. DOI: 10.1038/s41467-025-56796-9
Zhang X, Schlimgen RR, Singh S, Tomani MP, Volkman BF, Zhang C. (2024) Molecular basis for chemokine recognition and activation of XCR1. Proc Natl Acad Sci USA. 121, e2405732121. PMCID: PMC11621518. DOI: 10.1073/pnas.2405732121
Vergara S, Zhou X, Santiago U, Alaoui-El-Azher M, Conway JF, Sluis-Cremer N, Calero G. (2024) Structural basis of deoxynucleotide addition by HIV-1 RT during reverse transcription. Nat Commun. 15, 10553. PMCID: PMC11618517. DOI: 10.1038/s41467-024-54618-y
Werner Lass S, Smith BE, Camphire S, Eutsey RA, Prentice JA, Yerneni SS, Arun A, Bridges AA, Rosch JW, Conway JF, Campbell P, Hiller NL (2024) Pneumococcal extracellular vesicles mediate horizontal gene transfer via the transformation machinery. mSphere9: e0072724. PMCID: PMC11656791. DOI: 10.1128/msphere.00727-24
Hansen KH, Byeon CH, Liu Q, Drace T, Boesen T, Conway JF, Andreasen M, Akbey Ü. (2024) Structure of biofilm-forming functional amyloid PSMɑ1 from Staphylococcus aureus. Proc Natl Acad Sci USA 121, e2406775121. PMCID: PMC11331129. DOI: 10.1073/pnas.2406775121
Zhang X, Guseinov AA, Jenkins L, Li K, Tikhonova IG, Milligan G, Zhang C. (2024) Structural basis for the ligand recognition and signaling of free fatty acid receptors. Sci Adv. 10, eadj2384. PMCID: PMC10780892. DOI: 10.1126/sciadv.adj2384
Dalton MP, Cheng MH, Bahar I, Coleman JA. (2024)) Structural mechanisms for VMAT2 inhibition by tetrabenazine. eLife 12:RP91973. PMCID: PMC10959523. DOI: 10.7554/eLife.91973
Mittal A, Martin MF, Levin EJ, Adams C, Yang M, Provins L, Hall A, Procter M, Ledecq M, Hillisch A, Wolff C, Gillard M, Horanyi PS, Coleman JA. (2024) Structures of the synaptic vesicle protein 2A and 2B bound to anticonvulsants. Nat Struct Mol Biol 31, 1964-74. DOI: 10.1038/s41594-024-01335-1
Eren E, Watts NR, Conway JF, Wingfield PT. (2024) Myxococcus xanthus encapsulin cargo protein EncD is a flavin-binding protein with ferric reductase activity. Proc Natl Acad Sci USA 121, e2400426121. PMCID: PMC11126975. DOI: 10.1073/pnas.2400426121
Podgorski JM, Freeman K, Gosselin S, Huet A, Conway JF, Bird M, Grecco J, Patel S, Jacobs-Sera D, Hatfull G, Gogarten JP, Ravantti J, White SJ. (2023) A structural dendrogram of the actinobacteriophage major capsid proteins provides important structural insights into the evolution of capsid stability. Structure 22, S0969-2126. PMCID: PMC10071307. DOI: 10.1016/j.str.2022.12.012
Huet A, Oh B, Maurer J, Duda RL, Conway JF. (2023) A symmetry mismatch unraveled: How phage HK97 scaffold flexibly accommodates a 12-fold pore at a 5-fold viral capsid vertex. Sci Adv. 9, eadg8868. PMCID: PMC10275583. DOI: 10.1126/sciadv.adg8868
Zhang X, Weiß T, Cheng MH, Chen S, Ambrosius CK, Czerniak AS, Li K, Feng M, Bahar I, Beck-Sickinger AG, Zhang C. (2023) Structural basis of G protein-Coupled receptor CMKLR1 activation and signaling induced by a chemerin-derived agonist. PLoS Biol. 21, e3002188. PMCID: PMC10699647. DOI: 10.1371/journal.pbio.3002188
Tillman TS, Chen Q, Bondarenko V, Coleman JA, Xu Y, Tang P. (2023) SARS-CoV-2 Spike Protein Downregulates Cell Surface α7nAChR through a Helical Motif in the Spike Neck. ACS Chem Neurosci. 14, 689-698. PMCID: PMC9923440. DOI: 10.1021/acschemneuro.2c00610
Chen Y, Huang Y, Li Q, Luo Z, Zhang Z, Huang H, Sun J, Zhang L, Sun R, Bain D, Conway JF, Lu B & Li S. (2022) Targeting xkr8 via nanoparticles-mediated in situ codelivery of siRNA and chemotherapy drugs for cancer immunochemotherapy. Nat Nanotechnol. 18:193-204. PMCID: PMC9974593. DOI: 10.1038/s41565-022-01266-2
Fung HKH, Grimes S, Huet A, Duda RL, Chechik M, Gault J, Robinson CV, Hendrix RW, Jardine PJ, Conway JF, Baumann CG & Antson AA. (2022) Structural basis of DNA packaging by a ring-type ATPase from an archetypal viral system. Nucleic Acids Res 50, 8719–32. PMCID: PMC9410871. DOI: 10.1093/nar/gkac647
Sun D, Sang Z, Kim YJ, Xiang Y, Cohen T, Belford AK, Huet A, Conway JF, Sun J, Taylor DJ, Schneidman-Duhovny D, Zhang C, Huang W, Shi Y. (2021) Potent neutralizing nanobodies resist convergent circulating variants of SARS-CoV-2 by targeting diverse and conserved epitopes. Nat Commun, 12, 4676. PMCID: PMC8333356. DOI: 10.1038/s41467-021-24963-3
Goetschius DJ, Hartmann SR, Organtini LJ, Callaway H, Huang K, Bator CM, Ashley RE, Makhov AM, Conway JF, Parrish CR & Hafenstein SL (2021) High-resolution asymmetric structure of a Fab-virus complex reveals overlap with the receptor binding site. PNAS USA 118, e2025452118. PMCID: PMC8201801. DOI: 10.1073/pnas.2025452118
Campbell PL, Duda RL, Nassur J, Conway JF, Huet A. (2020) Mobile Loops and Electrostatic Interactions Maintain the Flexible Tail Tube of Bacteriophage Lambda. J Mol Biol. 432:384-395. PMC7575002. DOI: 10.1016/j.jmb.2019.10.031
Huet A, Huffman JB, Conway JF, Homa FL. (2020) J Virol. 94, e01534-20. Role of the Herpes Simplex Virus CVSC Proteins at the Capsid Portal Vertex. PMCID: PMC7925205. DOI: 10.1128/JVI.01534-20
Huet A, Duda RL, Boulanger P, Conway JF. (2019) Capsid expansion of bacteriophage T5 revealed by high resolution cryoelectron microscopy. Proc Natl Acad Sci USA 116:21037-21046. PMCID: PMC6800373. DOI: 10.1128/JVI.01534-20
Fu X, Ning J, Zhong Z, Ambrose Z, Charles Watkins S, Zhang P. (2019) AutoCLEM: An Automated Workflow for Correlative Live-Cell Fluorescence Microscopy and Cryo-Electron Tomography. Sci Rep. 9:19207. PMCID: PMC6915765. DOI: 10.1038/s41598-019-55766-8
Subramanian S, Maurer AC, Bator CM, Makhov AM, Conway JF, Turner KB, Marden JH, Vandenberghe LH & Hafenstein SL. (2019) Filling Adeno-Associated Virus Capsids: Estimating Success by Cryo-Electron Microscopy. Hum Gene Ther 30:1449-1460. PMCID: PMC6921155. DOI: 10.1089/hum.2019.041
Merg AD, Boatz JC, Mandal A, Zhao G, Mokashi-Punekar S, Liu C, Wang X, Zhang P, van der Wel PCA, Rosi NL (2016) Peptide-Directed Assembly of Single-Helical Gold Nanoparticle Superstructures Exhibiting Intense Chiroptical Activity. J Am Chem Soc. 2016 Oct 19;138(41):13655-13663. doi: 10.1021/jacs.6b07322. Epub 2016 Oct 11. PMCID: PMC5388601. DOI: 10.1021/jacs.6b07322
Vernhes E, Renouard M, Gilquin B, Cuniasse P, Durand D, England P, Hoos S, Huet A, Conway JF, Glukhov A, Ksenzenko V, Jacquet E, Nhiri N, Zinn-Justin S, Boulanger P. (2017) High affinity anchoring of the decoration protein pb10 onto the bacteriophage T5 capsid. Sci Rep 7: 41662. PMCID: PMC5292684. DOI: 10.1038/srep41662
Guan J, Bywaters SM, Brendle SA, Ashley RE, Makhov AM, Conway JF, Christensen ND, Hafenstein S. (2017) Cryoelectron Microscopy Maps of Human Papillomavirus 16 Reveal L2 Densities and Heparin Binding Site. Structure 25: 253-263. PMID: 28065506 DOI: 10.1016/j.str.2016.12.001
Guan J, Bywaters SM, Brendle SA, Ashley RE, Makhov AM, Conway JF, Christensen ND, Hafenstein S. (2017) High-Resolution Structure Analysis of Antibody V5 and U4 Conformational Epitopes on Human Papillomavirus 16. Viruses 9, E374. PMCID: PMC5744149. DOI: 10.3390/v9120374
Hua J, Huet A, Lopez CA, Toropova K, Pope WH, Duda RL, Hendrix RW, Conway JF. (2017) Capsids and Genomes of Jumbo-Sized Bacteriophages Reveal the Evolutionary Reach of the HK97 Fold. MBio 8, e01579-17. PMCID: PMC5646251. DOI: 10.1128/mBio.01579-17
Alvarez FJD, He S, Perilla JR, Jang S, Schulten K, Engelman AN, Scheres SHW, Zhang P. (2017) CryoEM structure of MxB reveals a novel oligomerization interface critical for HIV restriction. Sci Adv. 3, e1701264. PMCID: PMC5600524. DOI: 10.1126/sciadv.1701264
Subramanian S, Organtini LJ, Grossman A, Domeier PP, Cifuente JO, Makhov AM, Conway JF, D’Abramo A Jr, Cotmore SF, Tattersall P, Hafenstein S. (2017) Cryo-EM maps reveal five-fold channel structures and their modification by gatekeeper mutations in the parvovirus minute virus of mice (MVM) capsid. Virology 510, 216-223. PMCID: PMC5601314. DOI: 10.1016/j.virol.2017.07.015
Heming JD, Conway JF, Homa FL. (2017) Herpesvirus Capsid Assembly and DNA Packaging. Adv Anat Embryol Cell Biol. 223, 119-142. PMCID: PMC5548147. DOI: 10.1007/978-3-319-53168-7_6
Huffman JB, Daniel GR, Falck-Pedersen E, Huet A, Smith GA, Conway JF, Homa FL. (2017) The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores. J Virol. 91, e00641-17. PMCID: PMC5512264. DOI: 10.1128/JVI.00641-17
Huet A, Duda RL, Hendrix RW, Boulanger P, Conway JF. (2016) Correct Assembly of the Bacteriophage T5 Procapsid Requires Both the Maturation Protease and the Portal Complex. J Mol Biol. 428, 165-181. PMCID: PMC4738002. DOI: 10.1016/j.jmb.2015.11.019
Huet A, Makhov AM, Huffman JB, Vos M, Homa FL, Conway JF. (2016) Extensive subunit contacts underpin herpesvirus capsid stability and interior-to-exterior allostery. Nat Struct Mol Biol 23, 531-539. PMCID: PMC4899274. DOI: 10.1038/nsmb.3212
Organtini LJ, Lee H, Iketani S, Huang K, Ashley RE, Makhov AM, Conway JF, Parrish CR, Hafenstein S. (2016) Near-Atomic Resolution Structure of a Highly Neutralizing Fab Bound to Canine Parvovirus. J Virol 90: 9733-9742. PMCID: PMC5068525. DOI: 10.1128/JVI.01112-16
Lee H, Shingler KL, Organtini LJ, Ashley RE, Makhov AM, Conway JF, Hafenstein S. (2016) The novel asymmetric entry intermediate of a picornavirus captured with nanodiscs. Sci Adv 2: e1501929. PMCID: PMC4996645. DOI: 10.1126/sciadv.1501929
Organtini LJ, Shingler KL, Ashley RE, Capaldi EA, Durrani K, Dryden KA, Makhov AM, Conway JF, Pizzorno MC, Hafenstein S. (2017) Honey Bee Deformed Wing Virus Structures Reveal that Conformational Changes Accompany Genome Release. J Virol 91: e01795-16. PMCID: PMC5215330. DOI: 10.1128/JVI.01795-16
Ning J, Erdemci-Tandogan G, Yufenyuy EL, Wagner J, Himes BA, Zhao G, Aiken C, Zandi R, Zhang P. (2016) In vitro protease cleavage and computer simulations reveal the HIV-1 capsid maturation pathway. Nat Commun 7: 13689. PMCID: PMC5159922. DOI: 10.1038/ncomms13689
Other EM publications
Arya R, Ma D, Rempuszewski JJ, Sadaka NM, Frear AJ, Knapick B, Parker DM, Cooper VS, Van Tyne D, Shah NB, Doub JB, Akbey Ü, Fackler J, Basu S, Garber A, Nawrocki EM, Urish KL. (2026) Chronic suppression of a multidrug-resistant Pseudomonas aeruginosa in prosthetic joint infection using personalized bacteriophage treatment. Nat Commun 17, 9281. doi: PMCID: PMC13527055.. DOI: 10.1038/s41467-026-74326-z
Huang H, Mu Y, Yixian Y, Ji B, Wang Y, Chen C-Y, Chen Y, Luo Z, Li S, Zhang Z, Wang L, Conway JF, Yang D, Wang J, Sun J & Li S. Rational development of gemcitabine-based nanoplatform for targeting SERPINB9/Granzyme B axis to overcome chemo-immune-resistance. Nat Commun 16, 4176. (2025) PMCID: PMC12053578. DOI: 10.1038/s41467-025-59490-y
Vetturini AJ, Cagan J, Taylor RE. Generative design-enabled exploration of wireframe DNA origami nanostructures. Nucleic Acids Res 53:gkae1268 (2025). PMCID: PMC11754647. DOI: 10.1093/nar/gkae1268
Sun SY, Segev-Zarko LA, Pintilie GD, Kim CY, Staggers SR, Schmid MF, Egan ES, Chiu W, Boothroyd JC. Cryogenic electron tomography reveals novel structures in the apical complex of Plasmodium falciparum. mBio 15: e0286423 (2024). PMCID: PMC11005440. DOI: 10.1128/mbio.02864-23
Huang Y, Dong X, Sun SY, Lim TK, Lin Q, He CY. ARL3 GTPases facilitate ODA16 unloading from IFT in motile cilia. Sci Adv 10: eadq2950. (2024). PMCID: PMC11373600. DOI: 10.1126/sciadv.adq2950
Luo Z, Huang Y, Batra N, Chen Y, Huang H, Wang Y, Zhang Z, Li S, Chen CY, Wang Z, Sun J, Wang QJ, Yang D, Lu B, Conway JF, Li LY, Yu AM, Li S. Inhibition of iRhom1 by CD44-targeting nanocarrier for improved cancer immunochemotherapy. Nat Commun 15, 255 (2024). PMCID: PMC10766965. DOI: 10.1038/s41467-023-44572-6
Khare P, Edgecomb SX, Hamadani CM, Conway JF, Tanner EEL & Manickam DS. Ionic liquid-coated lipid nanoparticles increase siRNA uptake into CNS targets.. Nanoscale advances 6, 1853-1873, (2024). PMCID: PMC10964764. DOI: 10.1039/d3na00699a
Chen Y, Huang Y, Li Q, Luo Z, Zhang Z, Huang H, Sun J, Zhang L, Sun R, Bain DJ, Conway JF, Lu B, Li S. Targeting Xkr8 via nanoparticle-mediated in situ co-delivery of siRNA and chemotherapy drugs for cancer immunochemotherapy. Nat Nanotechnol 18, 193-204 (2023). PMCID: PMC9974593. DOI: 10.1038/s41565-022-01266-2
Khare P, Conway JF & Manickam DS. Lipidoid nanoparticles increase ATP uptake into hypoxic brain endothelial cells. European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (2022). DOI: 10.1016/j.ejpb.2022.10.011
Weiss S, Vergara S, Lin G & Calero G. Detection of Microcrystals for CryoEM. Methods in molecular biology (Clifton, N.J.) 2215, 299-307 (2021). PMCID: PMC8188421. DOI: 10.1007/978-1-0716-0966-8_14
Strubbe-Rivera JO, Schrad JR, Pavlov EV, Conway JF, Parent KN & Bazil JN. The mitochondrial permeability transition phenomenon elucidated by cryo-EM reveals the genuine impact of calcium overload on mitochondrial structure and function. Scientific reports 11, 1037 (2021). PMCID: PMC7806632. DOI: 10.1038/s41598-020-80398-8
Shen Z, Xiang Y, Vergara S, Chen A, Xiao Z, Santiago U, Jin C, Sang Z, Luo J, Chen K, Schneidman-Duhovny D, Camacho C, Calero G, Hu B & Shi Y. A resource of high-quality and versatile nanobodies for drug delivery. iScience 24, 103014 (2021). PMCID: PMC8426283. DOI: 10.1016/j.isci.2021.103014
Cassidy CK, Himes BA, Sun D, Ma J, Zhao G, Parkinson JS, Stansfeld PJ, Luthey-Schulten Z & Zhang P. Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations.. Commun Biol 3, 24 (2020). PMCID: PMC6954272. DOI: 10.1038/s42003-019-0748-0
Boatz JC, Piretra T, Lasorsa A, Matlahov I, Conway JF & van der Wel PCA. Protofilament Structure and Supramolecular Polymorphism of Aggregated Mutant Huntingtin Exon 1. JMol Biol 432, 4722-4744 (2020). PMCID: PMC8170701. DOI: 10.1016/j.jmb.2020.06.021
Wang Y, Palmfeldt J, Gregersen N, Makhov AM, Conway JF, Wang M, McCalley SP, Basu S, Alharbi H, St Croix C, Calderon MJ, Watkins S & Vockley J. Mitochondrial fatty acid oxidation and the electron transport chain comprise a multifunctional mitochondrial protein complex. J Biol Chem 294, 12380-12391 (2019). PMCID: PMC6699831. DOI: 10.1074/jbc.RA119.008680
Fu X, Ning J, Zhong Z, Ambrose Z, Charles Watkins S & Zhang P. AutoCLEM: An Automated Workflow for Correlative Live-Cell Fluorescence Microscopy and Cryo-Electron Tomography. Sci Rep 9, 19207 (2019). PMCID: PMC6915765. DOI: 10.1038/s41598-019-55766-8
Varlakhanova NV, Alvarez FJD, Brady TM, Tornabene BA, Hosford CJ, Chappie JS, Zhang P & Ford MGJ. Structures of the fungal dynamin-related protein Vps1 reveal a unique, open helical architecture. The Journal of cell biology 217, 3608-3624 (2018). DOI: 10.1083/jcb.201712021
Smith AN, Märker K, Piretra T, Boatz JC, Matlahov I, Kodali R, Hediger S, van der Wel PCA & De Paëpe G. Structural Fingerprinting of Protein Aggregates by Dynamic Nuclear Polarization-Enhanced Solid-State NMR at Natural Isotopic Abundance. Journal of the American Chemical Society 140, 14576-14580 (2018). DOI: 10.1021/jacs.8b09002
Ruhge LL, Huet AGE, Conway JF & Smith GA. The Apical Region of the Herpes Simplex Virus Major Capsid Protein Promotes Capsid Maturation. Journal of virology 92 (2018). DOI: 10.1128/JVI.00821-18
Drombosky KW, Rode S, Kodali R, Jacob TC, Palladino MJ & Wetzel R. Mutational analysis implicates the amyloid fibril as the toxic entity in Huntington’s disease. Neurobiology of disease 120, 126-138 (2018). DOI: 10.1016/j.nbd.2018.08.019
Chaudhary SC, Khalid S, Smethurst V, Monier D, Mobley J, Huet A, Conway JF & Napierala D. Proteomic profiling of extracellular vesicles released from vascular smooth muscle cells during initiation of phosphate-induced mineralization. Connective tissue research 59, 55-61 (2018). DOI: 10.1080/03008207.2018.1444759
Organtini LJ, Shingler KL, Ashley RE, Capaldi EA, Durrani K, Dryden KA, Makhov AM, Conway JF, Pizzorno MC & Hafenstein S. Honey Bee Deformed Wing Virus Structures Reveal that Conformational Changes Accompany Genome Release. Journal of virology 91 (2017). DOI: 10.1128/JVI.01795-16
Mandal A, Boatz JC, Wheeler TB & van der Wel PC. On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR. Journal of biomolecular NMR 67, 165-178 (2017). DOI: 10.1007/s10858-017-0089-6
Lin HK, Boatz JC, Krabbendam IE, Kodali R, Hou Z, Wetzel R, Dolga AM, Poirier MA & van der Wel PCA. Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core. Nature communications 8, 15462 (2017). DOI: 10.1038/ncomms15462
Kar K, Baker MA, Lengyel GA, Hoop CL, Kodali R, Byeon IJ, Horne WS, van der Wel PC & Wetzel R. Backbone Engineering within a Latent β-Hairpin Structure to Design Inhibitors of Polyglutamine Amyloid Formation. Journal of molecular biology 429, 308-323 (2017). DOI: 10.1016/j.jmb.2016.12.010
Huffman JB, Daniel GR, Falck-Pedersen E, Huet A, Smith GA, Conway JF & Homa FL. The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores. Journal of virology 91 (2017). DOI: 10.1128/JVI.00641-17
Boatz JC, Whitley MJ, Li M, Gronenborn AM & van der Wel PCA. Cataract-associated P23T γD-crystallin retains a native-like fold in amorphous-looking aggregates formed at physiological pH. Nature communications 8, 15137 (2017). DOI: 10.1038/ncomms15137
Tillman TS, Alvarez FJ, Reinert NJ, Liu C, Wang D, Xu Y, Xiao K, Zhang P & Tang P. Functional Human α7 Nicotinic Acetylcholine Receptor (nAChR) Generated from Escherichia coli. The Journal of biological chemistry 291, 18276-18282 (2016). DOI: 10.1074/jbc.M116.729970
Stevenson HP, Lin G, Barnes CO, Sutkeviciute I, Krzysiak T, Weiss SC, Reynolds S, Wu Y, Nagarajan V, Makhov AM, Lawrence R, Lamm E, Clark L, Gardella TJ, Hogue BG, Ogata CM, Ahn J, Gronenborn AM, Conway JF, Vilardaga JP, Cohen AE & Calero G. Transmission electron microscopy for the evaluation and optimization of crystal growth. Acta crystallographica. Section D, Structural biology 72, 603-615 (2016). PMCID: PMC4854312. DOI: 10.1107/S2059798316001546
Sahoo B, Arduini I, Drombosky KW, Kodali R, Sanders LH, Greenamyre JT & Wetzel R. Folding Landscape of Mutant Huntingtin Exon1: Diffusible Multimers, Oligomers and Fibrils, and No Detectable Monomer. PloS one 11, e0155747 (2016). DOI: 10.1371/journal.pone.0155747
Organtini LJ, Lee H, Iketani S, Huang K, Ashley RE, Makhov AM, Conway JF, Parrish CR & Hafenstein S. Near-Atomic Resolution Structure of a Highly Neutralizing Fab Bound to Canine Parvovirus. Journal of virology 90, 9733-9742 (2016). DOI: 10.1128/JVI.01112-16
Ning J, Erdemci-Tandogan G, Yufenyuy EL, Wagner J, Himes BA, Zhao G, Aiken C, Zandi R & Zhang P. In vitro protease cleavage and computer simulations reveal the HIV-1 capsid maturation pathway. Nature communications 7, 13689 (2016). DOI: 10.1038/ncomms13689
Misra P, Kodali R, Chemuru S, Kar K & Wetzel R. Rapid α-oligomer formation mediated by the Aβ C terminus initiates an amyloid assembly pathway. Nature communications 7, 12419 (2016). DOI: 10.1038/ncomms12419
Merg AD, Boatz JC, Mandal A, Zhao G, Mokashi-Punekar S, Liu C, Wang X, Zhang P, van der Wel PCA & Rosi NL. Peptide-Directed Assembly of Single-Helical Gold Nanoparticle Superstructures Exhibiting Intense Chiroptical Activity. Journal of the American Chemical Society 138, 13655-13663 (2016). DOI: 10.1021/jacs.6b07322
Huet A, Makhov AM, Huffman JB, Vos M, Homa FL & Conway JF. Extensive subunit contacts underpin herpesvirus capsid stability and interior-to-exterior allostery. Nature structural & molecular biology 23, 531-539 (2016). DOI: 10.1038/nsmb.3212
Huet A, Duda RL, Hendrix RW, Boulanger P & Conway JF. Correct Assembly of the Bacteriophage T5 Procapsid Requires Both the Maturation Protease and the Portal Complex. Journal of molecular biology 428, 165-181 (2016). DOI: 10.1016/j.jmb.2015.11.019
Hoop CL, Lin HK, Kar K, Magyarfalvi G, Lamley JM, Boatz JC, Mandal A, Lewandowski JR, Wetzel R & van der Wel PC. Huntingtin exon 1 fibrils feature an interdigitated β-hairpin-based polyglutamine core. Proceedings of the National Academy of Sciences of the United States of America 113, 1546-1551 (2016). DOI: 10.1073/pnas.1521933113
Chemuru S, Kodali R & Wetzel R. C-Terminal Threonine Reduces Aβ43 Amyloidogenicity Compared with Aβ42. Journal of molecular biology 428, 274-291 (2016). DOI: 10.1016/j.jmb.2015.06.008
Barnes CO, Kovaleva EG, Fu X, Stevenson HP, Brewster AS, DePonte DP, Baxter EL, Cohen AE & Calero G. Assessment of microcrystal quality by transmission electron microscopy for efficient serial femtosecond crystallography. Archives of biochemistry and biophysics 602, 61-68 (2016). DOI: 10.1016/j.abb.2016.02.011
Shingler KL, Cifuente JO, Ashley RE, Makhov AM, Conway JF & Hafenstein S. The enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro. Journal of virology 89, 1900-1908 (2015). PMCID PMC4300772. DOI: 10.1128/JVI.03098-14
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Cassidy CK, Himes BA, Alvarez FJ, Ma J, Zhao G, Perilla JR, Schulten K & Zhang P. CryoEM and computer simulations reveal a novel kinase conformational switch in bacterial chemotaxis signaling. eLife 4 (2015). DOI: 10.7554/eLife.08419
Albright BS, Kosinski A, Szczepaniak R, Cook EA, Stow ND, Conway JF & Weller SK. The putative herpes simplex virus 1 chaperone protein UL32 modulates disulfide bond formation during infection. Journal of virology 89, 443-453 (2015). DOI: 10.1128/JVI.01913-14
Zhao G & Zhang P. CryoEM analysis of capsid assembly and structural changes upon interactions with a host restriction factor, TRIM5α. Methods in molecular biology (Clifton, N.J.) 1087, 13-28 (2014). DOI: 10.1007/978-1-62703-670-2_2
Zhang L, Franks J, Stolz DB, Conway JF & Thibodeau PH. Inducible polymerization and two-dimensional assembly of the repeats-in-toxin (RTX) domain from the Pseudomonas aeruginosa alkaline protease. Biochemistry 53, 6452-6462 (2014). DOI: 10.1021/bi5007546
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Organtini LJ, Makhov AM, Conway JF, Hafenstein S & Carson SD. Kinetic and structural analysis of coxsackievirus B3 receptor interactions and formation of the A-particle. Journal of virology 88, 5755-5765 (2014). DOI: 10.1128/JVI.00299-14
Kar K, Arduini I, Drombosky KW, van der Wel PC & Wetzel R. D-polyglutamine amyloid recruits L-polyglutamine monomers and kills cells. Journal of molecular biology 426, 816-829 (2014). DOI: 10.1016/j.jmb.2013.11.019
Fu X, Himes BA, Ke D, Rice WJ, Ning J & Zhang P. Controlled bacterial lysis for electron tomography of native cell membranes. Structure (London, England : 1993) 22, 1875-1882 (2014). DOI: 10.1016/j.str.2014.09.017
Chemuru S, Kodali R & Wetzel R. Improved chemical synthesis of hydrophobic Aβ peptides using addition of C-terminal lysines later removed by carboxypeptidase B. Biopolymers 102, 206-221 (2014). DOI: 10.1002/bip.22470
Calero G, Cohen AE, Luft JR, Newman J & Snell EH. Identifying, studying and making good use of macromolecular crystals. Acta crystallographica. Section F, Structural biology communications 70, 993-1008 (2014). DOI: 10.1107/S2053230X14016574
Zhao G, Perilla JR, Yufenyuy EL, Meng X, Chen B, Ning J, Ahn J, Gronenborn AM, Schulten K, Aiken C & Zhang P. Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics. Nature 497, 643-646 (2013). DOI: 10.1038/nature12162
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Shingler KL, Yoder JL, Carnegie MS, Ashley RE, Makhov AM, Conway JF & Hafenstein S. The enterovirus 71 A-particle forms a gateway to allow genome release: a cryoEM study of picornavirus uncoating. PLoS pathogens 9, e1003240 (2013). DOI: 10.1371/journal.ppat.1003240
Preux O, Durand D, Huet A, Conway JF, Bertin A, Boulogne C, Drouin-Wahbi J, Trévarin D, Pérez J, Vachette P & Boulanger P. A two-state cooperative expansion converts the procapsid shell of bacteriophage T5 into a highly stable capsid isomorphous to the final virion head. Journal of molecular biology 425, 1999-2014 (2013). DOI: 10.1016/j.jmb.2013.03.002
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Mowrey DD, Cui T, Jia Y, Ma D, Makhov AM, Zhang P, Tang P & Xu Y. Open-channel structures of the human glycine receptor α1 full-length transmembrane domain. Structure (London, England : 1993) 21, 1897-1904 (2013). DOI: 10.1016/j.str.2013.07.014
Lee H, Cifuente JO, Ashley RE, Conway JF, Makhov AM, Tano Y, Shimizu H, Nishimura Y & Hafenstein S. A strain-specific epitope of enterovirus 71 identified by cryo-electron microscopy of the complex with fab from neutralizing antibody. Journal of virology 87, 11363-11370 (2013). DOI: 10.1128/JVI.01926-13
Kar K, Hoop CL, Drombosky KW, Baker MA, Kodali R, Arduini I, van der Wel PC, Horne WS & Wetzel R. β-hairpin-mediated nucleation of polyglutamine amyloid formation. Journal of molecular biology 425, 1183-1197 (2013). DOI: 10.1016/j.jmb.2013.01.016
Jun S, Zhao G, Ning J, Gibson GA, Watkins SC & Zhang P. Correlative microscopy for 3D structural analysis of dynamic interactions. Journal of visualized experiments : JoVE (2013). DOI: 10.3791/50386
Homa FL, Huffman JB, Toropova K, Lopez HR, Makhov AM & Conway JF. Structure of the pseudorabies virus capsid: comparison with herpes simplex virus type 1 and differential binding of essential minor proteins. Journal of molecular biology 425, 3415-3428 (2013). PMCID: PMC3779361. DOI: 10.1016/j.jmb.2013.06.034
Fang PA, Margolis HC, Conway JF, Simmer JP & Beniash E. CryoTEM study of effects of phosphorylation on the hierarchical assembly of porcine amelogenin and its regulation of mineralization in vitro. Journal of structural biology 183, 250-257 (2013). DOI: 10.1016/j.jsb.2013.05.011
Cifuente JO, Lee H, Yoder JD, Shingler KL, Carnegie MS, Yoder JL, Ashley RE, Makhov AM, Conway JF & Hafenstein S. Structures of the procapsid and mature virion of enterovirus 71 strain 1095. Journal of virology 87, 7637-7645 (2013). DOI: 10.1128/JVI.03519-12
Zhang L, Conway JF & Thibodeau PH. Calcium-induced folding and stabilization of the Pseudomonas aeruginosa alkaline protease. The Journal of biological chemistry 287, 4311-4322 (2012). DOI: 10.1074/jbc.M111.310300
Yeh JI, Levine AS, Du S, Chinte U, Ghodke H, Wang H, Shi H, Hsieh CL, Conway JF, Van Houten B & Rapić-Otrin V. Damaged DNA induced UV-damaged DNA-binding protein (UV-DDB) dimerization and its roles in chromatinized DNA repair. Proceedings of the National Academy of Sciences of the United States of America 109, E2737-2746 (2012). DOI: 10.1073/pnas.1110067109
Wang K, Strunk K, Zhao G, Gray JL & Zhang P. 3D structure determination of native mammalian cells using cryo-FIB and cryo-electron tomography. Journal of structural biology 180, 318-326 (2012). DOI: 10.1016/j.jsb.2012.07.003
Strunk KM, Wang K, Ke D, Gray JL & Zhang P. Thinning of large mammalian cells for cryo-TEM characterization by cryo-FIB milling. Journal of microscopy 247, 220-227 (2012). DOI: 10.1111/j.1365-2818.2012.03635.x
Mishra R, Jayaraman M, Roland BP, Landrum E, Fullam T, Kodali R, Thakur AK, Arduini I & Wetzel R. Inhibiting the nucleation of amyloid structure in a huntingtin fragment by targeting α-helix-rich oligomeric intermediates. Journal of molecular biology 415, 900-917 (2012). DOI: 10.1016/j.jmb.2011.12.011
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Jayaraman M, Kodali R, Sahoo B, Thakur AK, Mayasundari A, Mishra R, Peterson CB & Wetzel R. Slow amyloid nucleation via α-helix-rich oligomeric intermediates in short polyglutamine-containing huntingtin fragments. Journal of molecular biology 415, 881-899 (2012). DOI: 10.1016/j.jmb.2011.12.010
Toropova K, Huffman JB, Homa FL & Conway JF. The herpes simplex virus 1 UL17 protein is the second constituent of the capsid vertex-specific component required for DNA packaging and retention. Journal of virology 85, 7513-7522 (2011). PMCID: PMC3147944. DOI: 10.1128/JVI.00837-11
Szczepaniak R, Nellissery J, Jadwin JA, Makhov AM, Kosinski A, Conway JF & Weller SK. Disulfide bond formation contributes to herpes simplex virus capsid stability and retention of pentons. Journal of virology 85, 8625-8634 (2011). DOI: 10.1128/JVI.00214-11
Sivanandam VN, Jayaraman M, Hoop CL, Kodali R, Wetzel R & van der Wel PC. The aggregation-enhancing huntingtin N-terminus is helical in amyloid fibrils. Journal of the American Chemical Society 133, 4558-4566 (2011). DOI: 10.1021/ja110715f
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Li J, Hoop CL, Kodali R, Sivanandam VN & van der Wel PCA. Amyloid-like fibrils from a domain-swapping protein feature a parallel, in-register conformation without native-like interactions. The Journal of biological chemistry 286, 28988-28995 (2011). DOI: 10.1074/jbc.M111.261750
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Fang PA, Margolis HC, Conway JF, Simmer JP, Dickinson GH & Beniash E. Cryogenic transmission electron microscopy study of amelogenin self-assembly at different pH. Cells, tissues, organs 194, 166-170 (2011). DOI: 10.1159/000324250
Fang PA, Conway JF, Margolis HC, Simmer JP & Beniash E. Hierarchical self-assembly of amelogenin and the regulation of biomineralization at the nanoscale. Proceedings of the National Academy of Sciences of the United States of America 108, 14097-14102 (2011). DOI: 10.1073/pnas.1106228108
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Cockrell SK, Huffman JB, Toropova K, Conway JF & Homa FL. Residues of the UL25 protein of herpes simplex virus that are required for its stable interaction with capsids. Journal of virology 85, 4875-4887 (2011). DOI: 10.1128/JVI.00242-11
Ahn J, Novince Z, Concel J, Byeon CH, Makhov AM, Byeon IJ, Zhang P & Gronenborn AM. The Cullin-RING E3 ubiquitin ligase CRL4-DCAF1 complex dimerizes via a short helical region in DCAF1. Biochemistry 50, 1359-1367 (2011). DOI: 10.1021/bi101749s
Long KR, Yamamoto Y, Baker AL, Watkins SC, Coyne CB, Conway JF & Aridor M. Sar1 assembly regulates membrane constriction and ER export. The Journal of cell biology 190, 115-128 (2010). DOI: 10.1083/jcb.201004132
Lefterov I, Fitz NF, Cronican AA, Fogg A, Lefterov P, Kodali R, Wetzel R & Koldamova R. Apolipoprotein A-I deficiency increases cerebral amyloid angiopathy and cognitive deficits in APP/PS1DeltaE9 mice. The Journal of biological chemistry 285, 36945-36957 (2010). DOI: 10.1074/jbc.M110.127738
Kodali R, Williams AD, Chemuru S & Wetzel R. Abeta(1-40) forms five distinct amyloid structures whose beta-sheet contents and fibril stabilities are correlated. Journal of molecular biology 401, 503-517 (2010). DOI: 10.1016/j.jmb.2010.06.023
Huet A, Conway JF, Letellier L & Boulanger P. In vitro assembly of the T=13 procapsid of bacteriophage T5 with its scaffolding domain. Journal of virology 84, 9350-9358 (2010). DOI: 10.1128/JVI.00942-10
Conway JF, Cockrell SK, Copeland AM, Newcomb WW, Brown JC & Homa FL. Labeling and localization of the herpes simplex virus capsid protein UL25 and its interaction with the two triplexes closest to the penton. Journal of molecular biology 397, 575-586 (2010). DOI: 10.1016/j.jmb.2010.01.043
Thakur AK, Jayaraman M, Mishra R, Thakur M, Chellgren VM, Byeon IJ, Anjum DH, Kodali R, Creamer TP, Conway JF, Gronenborn AM & Wetzel R. Polyglutamine disruption of the huntingtin exon 1 N terminus triggers a complex aggregation mechanism. Nature structural & molecular biology 16, 380-389 (2009). DOI: 10.1038/nsmb.1570
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Chen CL, Zhang P & Rosi NL. A new peptide-based method for the design and synthesis of nanoparticle superstructures: construction of highly ordered gold nanoparticle double helices. Journal of the American Chemical Society 130, 13555-13557 (2008). DOI: 10.1021/ja805683r
Weigele PR, Pope WH, Pedulla ML, Houtz JM, Smith AL, Conway JF, King J, Hatfull GF, Lawrence JG & Hendrix RW. Genomic and structural analysis of Syn9, a cyanophage infecting marine Prochlorococcus and Synechococcus. Environmental microbiology 9, 1675-1695 (2007). DOI: 10.1111/j.1462-2920.2007.01285.x
Duda RL, Hendrix RW, Huang WM & Conway JF. Shared architecture of bacteriophage SPO1 and herpesvirus capsids. Current biology: CB 16, R11-13 (2006). DOI: 10.1016/j.cub.2005.12.023