Useful Links
RELION including tutorials: https://relion.readthedocs.io/en/release-5.0/
CryoSPARC: https://cryosparc.com
EMAN2 and tutorials:
https://blake.bcm.edu/emanwiki/EMAN2
https://blake.bcm.edu/emanwiki/EMAN2/Tutorials
crYOLO and tutorials:
https://cryolo.readthedocs.io/en/stable/
https://cryolo.readthedocs.io/en/stable/tutorials/tutorial_overview.html
Topaz: https://cb.csail.mit.edu/cb/topaz/
UCSF Chimera, ChimeraX, and tutorials:
https://www.rbvi.ucsf.edu/chimera/
https://www.cgl.ucsf.edu/chimera/tutorials.html
https://www.cgl.ucsf.edu/chimerax/
https://www.cgl.ucsf.edu/chimerax/tutorials.html
Cryo-EM University video series by Grant Jensen: https://em-learning.com/
A few interesting papers:
Frechard, Sharov, Werderer & Schultz P (2021) Methods Mol Biol. 2247, 243-256. doi: 10.1007/978-1-0716-1126-5_13. Optimization of Sample Preparation for the Observation of Macromolecular Complexes by Electron (cryo-)Microscopy.
Takizawa, Binshtein, Erwin, Pyburn, Mittendorf & Ohi (2017) Protein Sci. 26, 69-81. doi: 10.1002/pro.3054. While the revolution will not be crystallized, biochemistry reigns supreme.
Ohi, Li, Cheng & Walz (2004) Biol Proced Online 6, 23-34. doi: 10.1251/bpo70. Negative Staining and Image Classification – Powerful Tools in Modern Electron Microscopy.
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