Flow cytometry includes both analytical cytometry and cell sorting and is an extremely useful tool for a broad range of basic research projects. Cytometry can rapidly provide quantitative information regarding a wide variety of cellular properties, including DNA content, expression of surface, cytoplasmic, and nuclear antigens, receptor-ligand interactions, chromosome analysis, phagocytosis, target-effector cell binding, ion fluxes, expression of genes or gene products, and various light scattering properties. Another useful cytometry tool that we provide services for is multi-omic studies focusing on genomic and protein expression. The CF provides services that utilize Bruker’s NanoString platforms which generate bulk and digital spatial profiling (DSP) data, including single cell DSP data from the CosMx platform.
The CF offers several platforms:
Equipment
| Equipment Name | Description |
|---|---|
| BC CytoFlex 6L-Hillman | The CF operates three Beckman Coulter Cytoflex that present with different configurations. |
| BC Mosaic | |
| BD Fortessa I ( 4 laser )-Assembly | The Fortessa I is a four laser flow cytometer capable of detecting up to 15 distinct fluorochromes simultaneously. |
| BD Fortessa II ( 5 laser )-Hillman | The Fortessa II is a five laser flow cytometer capable of detecting up to 17 distinct fluorochromes simultaneously. |
| Beckman Coulter CytoFlex 2L | The CF operates three Beckman Coulter Cytoflex that present with different configurations. |
| Beckman Coulter CytoFlex 4L | The CF operates three Beckman Coulter Cytoflex that present with different configurations. |
| Beckman Coulter MoFlo Astrios | The Beckman Coulter MoFlo Astrios is a 5-laser, high-speed (10,000-20,000 cells/second) sorter capable of 6-way sorting, plate deposition, and single cell sorting. |
| CosMx Schedule | |
| Cytek Aurora Evo - Assembly | Cytek Aurora cytometerThe Cytek Aurora is a 5-laser, high-parameter spectral cytometer capable of detecting up to 30 distinct fluorochromes simultaneously. |
| ddPCR | Droplet Digital PCR (ddPCR) is an advanced molecular method that provides absolute quantification of nucleic acids (DNA or RNA) by partitioning a single sample into thousands of independent nanoliter-sized droplets. |
| GeoMx DSP | The GeoMx Digital Spatial Profiler (DSP) is an advanced spatial multiomics platform that measures RNA and protein expression within specific tissue regions. |
| nCounter | The nCounter Analysis System is an automated, digital multiplexing platform used to profile gene expression, protein abundance, and copy number variation without enzymatic amplification. |
| Sony MA900-Assembly | The Sony MA900 is a 4-laser, multi-application (5,000 cells/second) sorter capable of 4-way sorting and plate disposition. |
| Sony MA900-Hillman | The Sony MA900 is a 4-laser, multi-application (5,000 cells/second) sorter capable of 4-way sorting and plate disposition. |
| Thermo Bigfoot | The Invitrogen Bigfoot Spectral Cell Sorter is a high-speed, high-throughput flow cytometry instrument. |