About

The mission of the Hillman Cytometry Facility (CF) is to:

• Train investigators, their students and staff to use analytical cytometry to its fullest advantage in their research.
  • Basic and advanced training in the use of all facility cytometers is offered continuously.
  • The core facility is available to trained users 24 hours a day, seven days a week.
• Provide high quality cell separation by fluorescence activated cell sorting (FACS);
  • The CF provides both user-operated and staff-operated cell sorting service. Training for self-operated use of the Sony sorter is available. Staff assisted projects are run on the Thermo Bigfoot duel conventional / spectral high-speed sorter.
    • Facility is operated as a BSL-2+ facility and can sort live human cells and cells transfected with retroviral constructs.
• Operate and maintain the cytometers as well as the NanoString platforms.
• The multi-omics assays that the CF performs is a certified (by NanoString) service and as such, aids with project and study design, sample preparation, probe sequencing and facilitating avenues for data analysis.
• Consult on experimental design, analysis and assay development on flow cytometry and multi-omics studies, available to Hillman investigators without charge.

Location & Hours of Operation

Primary Location: Hillman Cancer Center Research Pavilion, Lab 1.45, 5117 Centre Avenue, Pittsburgh, PA 

Secondary Location: Assembly, Labs G530,G532, G536,  UPMC Hillman Cancer Center, 5150 Centre Avenue, Pittsburgh, PA

Citation Information

Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health under Award Number P30CA047904 and the UPMC Hillman Cancer Center Cancer Cytometry Facility (RRID:SCR_025361).