Services offered:
- Assistance for generating recombinant plasmids
- Design and implementation of CRISPR/Cas9 technology for in vivo and in vitro work
- Mouse and rat transgenesis, including transgenic and knockout/knockdown techniques using pronuclear injection, ES cell injection/chimera, and CRISPR/Cas9-mediated genome editing
- Production of lentivirus, adenovirus, and adeno-associated virus vectors
- Additional animal testing and procedures: teratoma and chimera testing to validate putative pluripotent stem cell lines, cryopreserve or re-derive of valuable transgenic lines (sperm or embryos)
- GETV services are approved by the Institutional Animal Care and Use Committee (IACUC), the recombinant DNA committee, and the environmental health and safety office of the University of Pittsburgh