The Cancer Genomics Facility (CGF) provides Hillman Cancer Center researchers with the following services:
Sample Pre-Processing
- Tissue Macro/Microdissection
DNA Services
- DNA Extraction and QA/QC
- DNA Library Preparation
RNA Services
- RNA Extraction and QA/QC
- RNA Library Preparation
DNA/RNA Services
- Sequencing
Equipment
| Equipment Name | Description |
|---|---|
| 10x Genomics CytAssist | The Visium CytAssist is a compact benchtop instrument by 10x Genomics that streamlines spatial transcriptomics and multiomics workflows |
| 10x Genomics Xenium | The Xenium platform by 10x Genomics is an advanced high-throughput spatial biology and single-cell in situ imaging system that maps gene and protein expression directly inside intact tissue sections. |
| Agilent Bioanalyzer 2100 | The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins, and cells on a single platform. |
| Biospec BeadBeater | The BioSpec BeadBeater is a high-energy laboratory homogenizer used to rapidly disrupt microorganisms, spores, and plant or animal tissues. |
| Covaris M220 | The Covaris M220 Focused-ultrasonicator is a compact, benchtop sample preparation instrument designed for precise DNA shearing, chromatin shearing, and nucleic acid fragmentation. |
| GE Ultrasound | GE HealthCare offers a diverse portfolio of advanced GE HealthCare Ultrasound Systems tailored to specific medical specialties and clinical environments. |
| Illumina MiSeq | 4-channel SBS chemistry. Ideal for targeted gene expression, miRNA, and small RNA analysis |
| Illumina NextSeq 500 | 2-channel SBS chemistry. Ideal for whole transcriptome, targeted gene expression, miRNA, and small RNA analysis |
| Leica Aperio CS2 | The Leica Biosystems Aperio CS2 is an ultra-compact desktop slide scanner built for fast, high-resolution digital pathology. |
| Logos Biosystems Luna-FX7 | The LUNA-FX7 Automated Cell Counter by Logos Biosystems is an advanced, high-throughput dual-fluorescence and brightfield automated cell counter designed for precision and regulatory compliance. |
| Miltenyi GentleMACS octo | The gentleMACS Octo Dissociator with Heaters is a benchtop automated instrument designed to process up to eight tissue samples in parallel for single-cell or single-nucleus isolation. |
| Thermo Scientific EVO 7000 | The EVOS M7000 Imaging System is a fully automated digital inverted benchtop microscope. |
| ThermoFisher Nanodrop | The Nanodrop (Thermo Scientific) is a micro-Volume UV-Vis spectrophotometer for nucleic acid and protein quantitation that allows for sample volumes as small as 0.5 μL. |
| ThermoFisher QuantStudio 3 | The QuantStudio 3 Real-Time PCR System is a PCR instrument designed for reliable gene expression analysis, genotyping, and pathogen detection. |
| ThermoFisher Qubit | The Qubit® 3.0 Fluorometer utilizes specifically designed fluorometric technology using Molecular Probes® dyes. These fluorescent dyes emit signals ONLY when bound to specific target molecules, even in the presence of free nucleotides or degraded nucleic acids. Qubit® fluorometric quantitation provides the most specific and sensitive DNA and RNA quantitation available, even at low concentrations. |