The SP8 is a fully automated upright laser scanning confocal microscope that is capable of tunable emission spectral detection. It is equipped with a Galvano and a Resonant scanner that allows both high resolution and fast speed imaging. The system has a wide range of objectives ranging from 10x to 100x. The extra CLARITY optics (25x objective) enable unprecedented levels of in-depth optical sectioning of thick tissue.
Capabilities:
- 2D and 3D imaging (with 3D reconstruction feature)
- Live and fixed sample (No environmental control)
- FRAP
- Line scan
- Tile scan /Large image stitching
- Multi-position imaging
Lasers (nm): 405, 488, 514, 552, 638
Scanners:
- Traditional Galvo scan
- 1k x 1k Resonant scan
Detectors:
- Two fluorescent PMTs
- Two HyD detectors (Faster and high sensitivity)
- One transmitted light PMT.
- Spectral Detection (Tunable emission wavelength)
Objectives:
| Name | Magnification & NA | Immersion | Location |
|---|---|---|---|
| HC PL APO | 10x 0.4NA CS2 | Dry | Microscope |
| HC PL APO | 20x 0.75NA CORR CS2 | IMM (Water/Oil/Gly) | Microscope |
| HCX APO | L 20x 1.0NA | Water (Dipping) | Cabinet |
| HCX IRAPO | L 25x 0.95NA | Water (Dipping) | Microscope |
| HC FLUOTAR | L 25x 1.0NA motCORR VISIR | IMM (Dipping) | Cabinet |
| HC PL APO | 40x 1.3NA CS2 | Oil | Microscope |
| HCX PL APO | 40x 0.85NA CORR CS | Dry | Cabinet |
| HC PL APO | 63x 1.4NA CS2 | Oil | Microscope |
| HCX APO | L 63x 0.9NA U-V-I | Water (Dipping) | Microscope |
| HC PL APO | 100x 1.44NA CORR CS | Oil | Cabinet |
Core Facility
Microscopy and Imaging Suite
Location
Lower Campus